Journal: Cancer cell
Article Title: Radiotherapy promotes cuproptosis and synergizes with cuproptosis inducers to overcome tumor radioresistance.
doi: 10.1016/j.ccell.2025.03.031
Figure Lengend Snippet: Figure 3. CTR1 upregulation and mitochondrial GSH depletion contribute to RT-mediated cuproptosis (A) Volcano plot from RNA sequencing (RNA-seq) analysis (DESeq2 R) of two untreated and three RT-treated samples showing the upregulation of CTR1 (also called SLC31A1) in FLO-1 cells 24 h after 6 Gy RT. (B and C) Relative CTR1 mRNA (B, n = 3) or protein (C) levels in FLO-1 or OE33 cells 24 h after RT treatment. (D) Immunohistochemical scoring of CTR1 in the indicated lung cancer PDX tumors with or without RT. n = 6 randomly selected magnification fields. (E and F) Relative CTR1 protein (E) or copper levels (F, n = 3) in FLO-1 or OE33 cells expressing empty vector (EV) or with CTR1 overexpression (oeCTR1). (legend continued on next page)
Article Snippet: Immunoprecipitation andWestern blot analysis were conducted as previously described.45,46 The primary antibodies and concentrations used in the current study included the following: MT1E (1:1,000, MA5-43655, Fisher), MT1X (1:1,000, 17172-1-AP, Proteintech), BACH1 (1:1,000, 14018-1-AP, Proteintech), NRF2 (1:1,000, 12721S, Cell Signaling), FDX1 (1:1,000, 12592-1-AP, Proteintech), LIAS (1:1,000, 11577-1-AP, Proteintech), Lipoic acid (1:1,000, 437695, Millipore), DLAT (1:1,000, 12362s, Cell Signaling), CTR1 (1:1,000, 13086, Cell Signaling), DMT1 (1:1,000, 20507-1-AP, Proteintech), ETS1 (1:2000, 14069, Cell Signaling), Vinculin (1:10000; V4505, Sigma), b-actin (1:5,000, 3700, Cell Signaling), Bcl-2 (1:1,000, 15071, Cell Signaling), SLC25A39 (1:1,000, 14963-1-AP, Proteintech), and Cleaved Caspase-3 (Asp175) (1:1000, 9661s, Cell Signaling).
Techniques: RNA Sequencing, Immunohistochemical staining, Expressing, Plasmid Preparation, Over Expression
Proteintech is a verified supplier 
